Nature Biotechnology
The expanding vistas of spatial transcriptomics
Nature Biotechnology, Published online: 03 October 2022; doi:10.1038/s41587-022-01448-2Spatial transcriptomics workflows, metrics and limitations are reviewed and discussed.
Nature Biotechnology
A language model beats alphafold2 on orphans
Nature Biotechnology, Published online: 03 October 2022; doi:10.1038/s41587-022-01466-0Protein structure prediction with a language model improves accuracy for orphan and designed proteins.
Nature Biotechnology
Innovating manufacturing technology in emerging economies
Nature Biotechnology, Published online: 28 September 2022; doi:10.1038/s41587-022-01499-5Innovating manufacturing technology in emerging economies
Nature Biotechnology
Real-time denoising enables high-sensitivity fluorescence time-lapse imaging beyond the shot-noise limit
Nature Biotechnology, Published online: 26 September 2022; doi:10.1038/s41587-022-01450-8DeepCAD-RT denoises fluorescence time-lapse images in real time.
Nature Biotechnology
Engineered CRISPR prime editors with compact, untethered reverse transcriptases
Nature Biotechnology, Published online: 26 September 2022; doi:10.1038/s41587-022-01473-1A split prime editor architecture facilitates screening and engineering of improved variants.
Nature Biotechnology
Haplotype-aware analysis of somatic copy number variations from single -cell transcriptomes
Nature Biotechnology, Published online: 26 September 2022; doi:10.1038/s41587-022-01468-yNumbat integrates expression, allele and haplotype information to detect copy number variations in scRNA-seq.
Nature Biotechnology
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites
Nature Biotechnology, Published online: 26 September 2022; doi:10.1038/s41587-022-01444-6A competitive CRISPR method discovers targets and phenotypic effects of the MYC oncogene.
Nature Biotechnology
Normalizing cancer RNA-seq data for library size, tumor purity and batch effects
Nature Biotechnology, Published online: 23 September 2022; doi:10.1038/s41587-022-01441-9Accurate identification and effective removal of unwanted variation is essential to derive meaningful biological results from large and complex RN…
Nature Biotechnology
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins
Nature Biotechnology, Published online: 22 September 2022; doi:10.1038/s41587-022-01456-2KineTACs are modular bispecific antibodies for degradation of extracellular and cell-surface proteins.
Nature Biotechnology
Scalable in situ single-cell profiling by electrophoretic capture of mRNA using EEL FISH
Nature Biotechnology, Published online: 22 September 2022; doi:10.1038/s41587-022-01455-3EEL FISH profiles spatial transcriptomes in single cells in large, complex tissue samples.