Chromosomes and Gene Expression

Target binding triggers hierarchical phosphorylation of human Argonaute-2 to promote target release

Argonaute (Ago) proteins play a central role in post-transcriptional gene regulation through RNA interference (RNAi). Agos bind small RNAs (sRNAs) including small interfering RNAs (siRNAs) and microRNAs (miRNAs) to form the functional core of the RNA-i…

By Brianna Bibel, 3 years ago

Cell Biology

The TFIIH complex is required to establish and maintain mitotic chromosome structure

Condensins compact chromosomes to promote their equal segregation during mitosis, but the mechanism of condensin engagement with and action on chromatin is incompletely understood. Here, we show that the general transcription factor TFIIH complex is co…

By Alexander E Kelly, 3 years ago

Cell Biology

Quantification of protein abundance and interaction defines a mechanism for operation of the circadian clock

The mammalian circadian clock exerts control of daily gene expression through cycles of DNA binding. Here we develop a quantitative model of how a finite pool of BMAL1 protein can regulate thousands of target sites over daily time scales. We used quant…

By Alex Ashton Koch, 3 years ago